Signalling

Part:BBa_K2070020

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)


Cell division dependent GFP generator

This part contains BBa_E0240 ligated downstream of promoter Pnrd (BBa_K847211). The promoter is only activated during cell division.



Usage and Biology

Measurement of nrd Promoter Activity. The fluorescence from the Pnrd promoter (ligated with BBa_E0240) is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 904


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